Reagents

Calcium Chloride (CaCl₂) – 1 M

Cholesterol – 10 mg/ml

Diluted DAPI Solution

Ethylenedinitrilotetraacetic acid (EDTA) – 0.5M, pH 8

KP Buffer

Magnesium Sulfate (MgSO₄)- 1 M

Phosphate Buffered Saline (PBS) – 1X/10X

Phosphate Buffered Solution 0.1%/0.01% Tween (PBT)

S Buffer

Sodium Hydroxide (NaOH) – 10N