S BufferReagents (for 1L): 0.05 M K2HPO4 – 129 mL 0.05 M KH2PO4 – 871 mL 5.85 g NaCl Protocol: Dissolve the NaCl in the 0.05 M K2HPO4 and the 0.05 M KH2PO4 solution. Mix thoroughly.